Fractionation of the major whey proteins and isolation of b-Lactoglobulin variants by anion exchange chromatography
نویسندگان
چکیده
A method for the separation and fractionation of the major whey proteins from a whey protein concentrate (WPC80) by anion-exchange chromatography coupled to a Fast Protein Liquid Chromatography (FPLC) system is proposed. The method is based on the use of an ionic column (Mono Q) and a salt gradient elution by increasing the ionic strength of the elution buffer (Tris–HCl 20 mM plus 0 to 1 M NaCl). The proposed method was found to be suitable to fractionate the major whey proteins from theWPC80 in different fractions, namely one fraction containing all the a-Lactalbumin and immunoglobulins; another fraction containing all the bovine serum albumin; and two distinct fractions each containing a different variant of b-Lactoglobulin. A 60.5% (w/w) recovery of the two main b-Lactoglobulin variants was
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Beta-lactoglobulin (BLG) is a major whey protein in the milk of ruminants, e.g. cows, and such non-ruminants as horses. This protein is resistant under various conditions of pH and temperature. There is some evidence of absorption of some intact BLG in humans. Infants being fed with cow’...
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